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PCR Biosystems
PCR Reagents for Research & Diagnostics:
PCR Biosystems develops a range of PCR reagents that maximise yield and sensitivity from the simplest to most challenging of reactions. Their products outperform every competitor in terms of yield, specificity, sensitivity, and speed. Applications include PCR, qPCR, NGS, Molecular Diagnostics, cDNA Synthesis, and DNA Extraction.
UltraScript cDNA Synthesis Kit Separate Oligos
£ 339.00 ex. VATcDNA Synthesis Pack size 100 reactions The enzyme allows highly sensitive reverse transcription of viral RNA sequences, making it an ideal component of RT-qPCR-based detection tests. Available as a stand-alone enzyme, or as a ready mix comprising reverse transcriptase, buffer system and, optionally, with an optimized blend of random hexamers with anchored oligo(dT) primers. This reverse transcriptase provides unbiased, efficient and sensitive cDNA synthesis over a broad range of RNA template concentrations. Features. Unbiased representation of 5′ and 3′ mRNA transcript ends, Reduced RNase H activity, Thermostable reverse transcriptase 45°C to 55°C, Advanced RNase inhibitor, High cDNA yields from as little as 4 pg total RNA, Accurate reverse transcription of GC-rich templates, Sensitive detection of low copy number transcripts, Advanced buffer chemistry including Mg and dNTPs, Both premixed and separate oligo cDNA synthesis kits available.
UltraScript Reverse Transcriptase
£ 168.00 ex. VATcDNA Synthesis Pack size 10,000 units The enzyme allows highly sensitive reverse transcription of viral RNA sequences, making it an ideal component of RT-qPCR-based detection tests. Available as a stand-alone enzyme, or as a ready mix comprising reverse transcriptase, buffer system and, optionally, with an optimized blend of random hexamers with anchored oligo(dT) primers. This reverse transcriptase provides unbiased, efficient and sensitive cDNA synthesis over a broad range of RNA template concentrations. Features. Unbiased representation of 5’and 3’mRNA transcript ends, Reduced RNase H activity, Thermostable reverse transcriptase 45°C to 55°C, Advanced RNase inhibitor, High cDNA yields from as little as 4 pg total RNA, Accurate reverse transcription of GC-rich templates, Sensitive detection of low copy number transcripts, Advanced buffer chemistry including Mg and dNTPs, Both premixed and separate oligo cDNA synthesis kits available.
UltraScript Reverse Transcriptase
£ 607.00 ex. VATcDNA Synthesis Pack size 40,000 units The enzyme allows highly sensitive reverse transcription of viral RNA sequences, making it an ideal component of RT-qPCR-based detection tests. Available as a stand-alone enzyme, or as a ready mix comprising reverse transcriptase, buffer system and, optionally, with an optimized blend of random hexamers with anchored oligo(dT) primers. This reverse transcriptase provides unbiased, efficient and sensitive cDNA synthesis over a broad range of RNA template concentrations. Features. Unbiased representation of 5′ and 3’mRNA transcript ends, Reduced RNase H activity, Thermostable reverse transcriptase 45°C to 55°C, Advanced RNase inhibitor, High cDNA yields from as little as 4 pg total RNA, Accurate reverse transcription of GC-rich templates, Sensitive detection of low copy number transcripts, Advanced buffer chemistry including Mg and dNTPs, Both premixed and separate oligo cDNA synthesis kits available.
VeriFi Hot Start Mix
£ 167.00 ex. VATLong range and high fidelity PCR with AptaLock Pack size 100 reactions Improved DNA binding and increased processivity give significant improvements in speed, yield, and sensitivity while also increasing PCR success rates of long and challenging templates. VeriFi Hot Start Polymerase was formerly known as PCRBIO HS VeriFi Polymerase. Features. AptaLock? hot start technology for maximized sensitivity and specificity, Greater success with long and/or GC or AT-rich templates (17.5 kb and over), High-temperature cycling ? up to 100?C denaturation to better separate GC-rich sequences, 100x higher fidelity than Taq DNA polymerase, Room temperature setup, Reaction mix stability for up to 24 hours both before and after PCR run, Generates blunt-end PCR products, Also available as a 2x ready mix with the option of a red dye for direct gel loading.
VeriFi Hot Start Mix
£ 648.00 ex. VATLong range and high fidelity PCR with AptaLock Pack size 500 reactions Improved DNA binding and increased processivity give significant improvements in speed, yield, and sensitivity while also increasing PCR success rates of long and challenging templates. VeriFi Hot Start Polymerase was formerly known as PCRBIO HS VeriFi Polymerase. Features. AptaLock? hot start technology for maximized sensitivity and specificity, Greater success with long and/or GC or AT-rich templates (17.5 kb and over), High-temperature cycling ? up to 100?C denaturation to better separate GC-rich sequences, 100x higher fidelity than Taq DNA polymerase, Room temperature setup, Reaction mix stability for up to 24 hours both before and after PCR run, Generates blunt-end PCR products, Also available as a 2x ready mix with the option of a red dye for direct gel loading.
VeriFi Hot Start Mix Red
£ 167.00 ex. VATLong range and high fidelity PCR with AptaLock (red loading dye) Pack size 100 reactions Improved DNA binding and increased processivity give significant improvements in speed, yield, and sensitivity while also increasing PCR success rates of long and challenging templates. VeriFi Hot Start Polymerase was formerly known as PCRBIO HS VeriFi Polymerase. Features. AptaLock? hot start technology for maximized sensitivity and specificity, Greater success with long and/or GC or AT-rich templates (17.5 kb and over), High-temperature cycling ? up to 100?C denaturation to better separate GC-rich sequences, 100x higher fidelity than Taq DNA polymerase, Room temperature setup, Reaction mix stability for up to 24 hours both before and after PCR run, Generates blunt-end PCR products, Also available as a 2x ready mix with the option of a red dye for direct gel loading.
VeriFi Hot Start Mix Red
£ 648.00 ex. VATLong range and high fidelity PCR with AptaLock (red loading dye) Pack size 500 reactions Improved DNA binding and increased processivity give significant improvements in speed, yield, and sensitivity while also increasing PCR success rates of long and challenging templates. VeriFi Hot Start Polymerase was formerly known as PCRBIO HS VeriFi Polymerase. Features. AptaLock? hot start technology for maximized sensitivity and specificity, Greater success with long and/or GC or AT-rich templates (17.5 kb and over), High-temperature cycling ? up to 100?C denaturation to better separate GC-rich sequences, 100x higher fidelity than Taq DNA polymerase, Room temperature setup, Reaction mix stability for up to 24 hours both before and after PCR run, Generates blunt-end PCR products, Also available as a 2x ready mix with the option of a red dye for direct gel loading.
VeriFi Hot Start Polymerase
£ 98.00 ex. VATLong range and high fidelity PCR with AptaLock (incl. dNTPs) Pack size 100 units Improved DNA binding and increased processivity give significant improvements in speed, yield, and sensitivity while also increasing PCR success rates of long and challenging templates. VeriFi Hot Start Polymerase was formerly known as PCRBIO HS VeriFi Polymerase. Features. AptaLock? hot start technology for maximized sensitivity and specificity, Greater success with long and/or GC or AT-rich templates (17.5 kb and over), High-temperature cycling ? up to 100?C denaturation to better separate GC-rich sequences, 100x higher fidelity than Taq DNA polymerase, Room temperature setup, Reaction mix stability for up to 24 hours both before and after PCR run, Generates blunt-end PCR products, Also available as a 2x ready mix with the option of a red dye for direct gel loading.
VeriFi Hot Start Polymerase
£ 402.00 ex. VATLong range and high fidelity PCR with AptaLock (incl. dNTPs) Pack size 500 units Improved DNA binding and increased processivity give significant improvements in speed, yield, and sensitivity while also increasing PCR success rates of long and challenging templates. VeriFi Hot Start Polymerase was formerly known as PCRBIO HS VeriFi Polymerase. Features. AptaLock? hot start technology for maximized sensitivity and specificity, Greater success with long and/or GC or AT-rich templates (17.5 kb and over), High-temperature cycling ? up to 100?C denaturation to better separate GC-rich sequences, 100x higher fidelity than Taq DNA polymerase, Room temperature setup, Reaction mix stability for up to 24 hours both before and after PCR run, Generates blunt-end PCR products, Also available as a 2x ready mix with the option of a red dye for direct gel loading.
VeriFi Library Amplification Mix
£ 120.00 ex. VATLibrary amplification Pack size 50 reactions A superior proofreading Pfu polymerase in a specially formulated 2x PCR ready mix designed for NGS library amplification with reduced GC bias1. This cutting-edge PCR mix offers market leading performance enabling the acquisition of superior quality datasets with a higher number of unique reads2. The mix can be combined with our library quantification kit during NGS library preparation. Features. Low GC bias, ideal for high GC/AT targets, more unique reads per NGS dataset for superior data quality, AptaLock? hot start technology for maximum sensitivity and specificity, 100x higher fidelity than Taq DNA polymerase, Room temperature setup, 2x ready mix for minimal pipetting.
VeriFi Library Amplification Mix
£ 520.00 ex. VATLibrary amplification Pack size 250 reactions A superior proofreading Pfu polymerase in a specially formulated 2x PCR ready mix designed for NGS library amplification with reduced GC bias1. This cutting-edge PCR mix offers market leading performance enabling the acquisition of superior quality datasets with a higher number of unique reads2. The mix can be combined with our library quantification kit during NGS library preparation. Features. Low GC bias, ideal for high GC/AT targets, more unique reads per NGS dataset for superior data quality, AptaLock? hot start technology for maximum sensitivity and specificity, 100x higher fidelity than Taq DNA polymerase, Room temperature setup, 2x ready mix for minimal pipetting.
VeriFi Mix
£ 519.00 ex. VATLong range and high fidelity PCR Pack size 500 reactions Improved DNA binding and increased processivity result in shorter extension times, higher yields, and the ability to amplify longer and more difficult targets. VeriFi Polymerase was formerly known as PCRBIO VeriFi Polymerase. Features. High-temperature cycling ? up to 100 ?C denaturation to better separate GC-rich templates, Increased PCR success rates with complex genomic templates (17.5 kb and over), High yields under standard and fast PCR conditions (10-30 s/kb), Efficient and specific amplification from challenging templates including GC and AT-rich sequences, 100x higher fidelity than Taq DNA polymerase, Generates blunt-end PCR products, Available as a convenient 2x ready mix with the option of a red dye for direct gel loading.
VeriFi Mix
£ 134.00 ex. VATLong range and high fidelity PCR Pack size 100 reactions Improved DNA binding and increased processivity result in shorter extension times, higher yields, and the ability to amplify longer and more difficult targets. VeriFi Polymerase was formerly known as PCRBIO VeriFi Polymerase. Features. High-temperature cycling ? up to 100 ?C denaturation to better separate GC-rich templates, Increased PCR success rates with complex genomic templates (17.5 kb and over), High yields under standard and fast PCR conditions (10-30 s/kb), Efficient and specific amplification from challenging templates including GC and AT-rich sequences, 100x higher fidelity than Taq DNA polymerase, Generates blunt-end PCR products, Available as a convenient 2x ready mix with the option of a red dye for direct gel loading.
VeriFi Mix Red
£ 134.00 ex. VATLong range and high fidelity PCR, direct gel loading Pack size 100 reactions Improved DNA binding and increased processivity result in shorter extension times, higher yields, and the ability to amplify longer and more difficult targets. VeriFi Polymerase was formerly known as PCRBIO VeriFi Polymerase. Features. High-temperature cycling ? up to 100 ?C denaturation to better separate GC-rich templates, Increased PCR success rates with complex genomic templates (17.5 kb and over), High yields under standard and fast PCR conditions (10-30 s/kb), Efficient and specific amplification from challenging templates including GC and AT-rich sequences, 100x higher fidelity than Taq DNA polymerase, Generates blunt-end PCR products, Available as a convenient 2x ready mix with the option of a red dye for direct gel loading.
VeriFi Mix Red
£ 519.00 ex. VATLong range and high fidelity PCR, direct gel loading Pack size 500 reactions Improved DNA binding and increased processivity result in shorter extension times, higher yields, and the ability to amplify longer and more difficult targets. VeriFi Polymerase was formerly known as PCRBIO VeriFi Polymerase. Features. High-temperature cycling ? up to 100 ?C denaturation to better separate GC-rich templates, Increased PCR success rates with complex genomic templates (17.5 kb and over), High yields under standard and fast PCR conditions (10-30 s/kb), Efficient and specific amplification from challenging templates including GC and AT-rich sequences, 100x higher fidelity than Taq DNA polymerase, Generates blunt-end PCR products, Available as a convenient 2x ready mix with the option of a red dye for direct gel loading.
VeriFi Polymerase
£ 84.00 ex. VATLong range and high fidelity PCR (incl. dNTPs) Pack size 100 units Improved DNA binding and increased processivity result in shorter extension times, higher yields, and the ability to amplify longer and more difficult targets. VeriFi Polymerase was formerly known as PCRBIO VeriFi Polymerase. Features. High-temperature cycling ? up to 100 ?C denaturation to better separate GC-rich templates, Increased PCR success rates with complex genomic templates (17.5 kb and over), High yields under standard and fast PCR conditions (10-30 s/kb), Efficient and specific amplification from challenging templates including GC and AT-rich sequences, 100x higher fidelity than Taq DNA polymerase, Generates blunt-end PCR products, Available as a convenient 2x ready mix with the option of a red dye for direct gel loading.
VeriFi Polymerase
£ 335.00 ex. VATLong range and high fidelity PCR (incl. dNTPs) Pack size 500 units Improved DNA binding and increased processivity result in shorter extension times, higher yields, and the ability to amplify longer and more difficult targets. VeriFi Polymerase was formerly known as PCRBIO VeriFi Polymerase. Features. High-temperature cycling ? up to 100 ?C denaturation to better separate GC-rich templates, Increased PCR success rates with complex genomic templates (17.5 kb and over), High yields under standard and fast PCR conditions (10-30 s/kb), Efficient and specific amplification from challenging templates including GC and AT-rich sequences, 100x higher fidelity than Taq DNA polymerase, Generates blunt-end PCR products, Available as a convenient 2x ready mix with the option of a red dye for direct gel loading.