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PCR Biosystems
PCR Reagents for Research & Diagnostics:
PCR Biosystems develops a range of PCR reagents that maximise yield and sensitivity from the simplest to most challenging of reactions. Their products outperform every competitor in terms of yield, specificity, sensitivity, and speed. Applications include PCR, qPCR, NGS, Molecular Diagnostics, cDNA Synthesis, and DNA Extraction.
qPCRBIO SyGreen Mix Lo-ROX
£ 1,284.00 ex. VATSyGreen real-time PCR – 50 mL bottle Pack size 5000 x 20 ?L rxns qPCRBIO SyGreen Mix can be used to reliably quantify any DNA template including genomic, cDNA and viral sequences using dye-based qPCR, and is able to detect extremely low copy number targets with the highest efficiency. Features. Rapid extension rate for early Ct values, Market-leading sensitivity ? increased limit of detection, Specific amplification from complex templates including GC-rich sequences, non-PCR inhibiting intercalating dye, also available as an easy-to-see blue mix, Compatible with all standard and fast cycling real-time instruments.
qPCRBIO SyGreen Mix Lo-ROX
£ 1,490.00 ex. VATSyGreen real-time PCR – 50 x 1 mL pouch Pack size 5000 x 20 ?L rxns qPCRBIO SyGreen Mix can be used to reliably quantify any DNA template including genomic, cDNA and viral sequences using dye-based qPCR, and is able to detect extremely low copy number targets with the highest efficiency. Features. Rapid extension rate for early Ct values, Market-leading sensitivity ? increased limit of detection, Specific amplification from complex templates including GC-rich sequences, non-PCR inhibiting intercalating dye, also available as an easy-to-see blue mix, Compatible with all standard and fast cycling real-time instruments.
qPCRBIO SyGreen Mix Separate-ROX
£ 40.00 ex. VATSyGreen real-time PCR – No- Lo- Hi- ROX Pack size 100 x 20 ?L rxns qPCRBIO SyGreen Mix can be used to reliably quantify any DNA template including genomic, cDNA and viral sequences using dye-based qPCR, and is able to detect extremely low copy number targets with the highest efficiency. Features. Rapid extension rate for early Ct values, Market-leading sensitivity ? increased limit of detection, Specific amplification from complex templates including GC-rich sequences, non-PCR inhibiting intercalating dye, also available as an easy-to-see blue mix, Compatible with all standard and fast cycling real-time instruments.
qPCRBIO SyGreen Mix Separate-ROX
£ 189.00 ex. VATSyGreen real-time PCR – No- Lo- Hi- ROX Pack size 500 x 20 ?L rxns qPCRBIO SyGreen Mix can be used to reliably quantify any DNA template including genomic, cDNA and viral sequences using dye-based qPCR, and is able to detect extremely low copy number targets with the highest efficiency. Features. Rapid extension rate for early Ct values, Market-leading sensitivity ? increased limit of detection, Specific amplification from complex templates including GC-rich sequences, non-PCR inhibiting intercalating dye, also available as an easy-to-see blue mix, Compatible with all standard and fast cycling real-time instruments.
qPCRBIO SyGreen Mix Separate-ROX
£ 642.00 ex. VATSyGreen real-time PCR – No- Lo- Hi- ROX Pack size 2000 x 20 ?L rxns qPCRBIO SyGreen Mix can be used to reliably quantify any DNA template including genomic, cDNA and viral sequences using dye-based qPCR, and is able to detect extremely low copy number targets with the highest efficiency. Features. Rapid extension rate for early Ct values, Market-leading sensitivity ? increased limit of detection, Specific amplification from complex templates including GC-rich sequences, non-PCR inhibiting intercalating dye, also available as an easy-to-see blue mix, Compatible with all standard and fast cycling real-time instruments.
qPCRBIO SyGreen Mix Separate-ROX
£ 1,284.00 ex. VATSyGreen real-time PCR – 50 mL bottle Pack size 5000 x 20 ?L rxns qPCRBIO SyGreen Mix can be used to reliably quantify any DNA template including genomic, cDNA and viral sequences using dye-based qPCR, and is able to detect extremely low copy number targets with the highest efficiency. Features. Rapid extension rate for early Ct values, Market-leading sensitivity ? increased limit of detection, Specific amplification from complex templates including GC-rich sequences, non-PCR inhibiting intercalating dye, also available as an easy-to-see blue mix, Compatible with all standard and fast cycling real-time instruments.
qPCRBIO SyGreen Mix Separate-ROX
£ 1,490.00 ex. VATSyGreen real-time PCR – 50 x 1 mL pouch Pack size 5000 x 20 ?L rxns qPCRBIO SyGreen Mix can be used to reliably quantify any DNA template including genomic, cDNA and viral sequences using dye-based qPCR, and is able to detect extremely low copy number targets with the highest efficiency. Features. Rapid extension rate for early Ct values, Market-leading sensitivity ? increased limit of detection, Specific amplification from complex templates including GC-rich sequences, non-PCR inhibiting intercalating dye, also available as an easy-to-see blue mix, Compatible with all standard and fast cycling real-time instruments.
RiboShield RNase Inhibitor
£ 83.00 ex. VATRNase Inhibition Pack size 2,500 units This RNase inhibitor is designed for use in RNA-sensitive applications such as RT-qPCR, cDNA synthesis and RNA sequencing, where the presence of even small amounts of RNase can be highly detrimental to RNA quality and experimental outcome. Feature. Superior protection leading to better performance of RNA-sensitive applications, particularly suited to incorporation into saliva-based tests for SARS-CoV-2 detection, Compatible with reverse transcriptase, RNA polymerases and Taq DNA polymerase, Protects RNA samples during storage, inhibits eukaryotic RNases, including RNase A, B and C, Stable up to 65°C for at least 30 minutes, Ribonuclease and phosphatase free.
RiboShield RNase Inhibitor
£ 296.00 ex. VATRNase Inhibition Pack size 10,000 units This RNase inhibitor is designed for use in RNA-sensitive applications such as RT-qPCR, cDNA synthesis and RNA sequencing, where the presence of even small amounts of RNase can be highly detrimental to RNA quality and experimental outcome. Feature. Superior protection leading to better performance of RNA-sensitive applications, particularly suited to incorporation into saliva-based tests for SARS-CoV-2 detection, Compatible with reverse transcriptase, RNA polymerases and Taq DNA polymerase, Protects RNA samples during storage, inhibits eukaryotic RNases, including RNase A, B and C, Stable up to 65°C for at least 30 minutes, Ribonuclease and phosphatase free.
UltraScript 2.0 cDNA Synthesis Kit
£ 113.00 ex. VATcDNA Synthesis Pack size 25 reactions This modified MMLV reverse transcriptase can be used with reaction temperatures of over 55°C, giving improved specificity, higher cDNA yields and more full-length cDNA product. Features. Highly thermostable reverse transcriptase 55°C to 65°C and above, Advanced RNase inhibitor, High cDNA yields from as little as 20pg total RNA, Accurate reverse transcription of GC-rich and highly structured transcripts, High sensitivity for challenging templates, Reduced RNase H activity, Available as a stand-alone enzyme with buffer, a cDNA synthesis kit with premixed oligos and a cDNA synthesis kit with separate oligos.
UltraScript 2.0 cDNA Synthesis Kit
£ 383.00 ex. VATcDNA Synthesis Pack size 100 reactions This modified MMLV reverse transcriptase can be used with reaction temperatures of over 55°C, giving improved specificity, higher cDNA yields and more full-length cDNA product. Features. Highly thermostable reverse transcriptase 55°C to 65°C and above, Advanced RNase inhibitor, High cDNA yields from as little as 20pg total RNA, Accurate reverse transcription of GC-rich and highly structured transcripts, High sensitivity for challenging templates, Reduced RNase H activity, Available as a stand-alone enzyme with buffer, a cDNA synthesis kit with premixed oligos and a cDNA synthesis kit with separate oligos.
UltraScript 2.0 cDNA Synthesis Kit Separate Oligos
£ 113.00 ex. VATcDNA Synthesis Pack size 25 reactions This modified MMLV reverse transcriptase can be used with reaction temperatures of over 55°C, giving improved specificity, higher cDNA yields and more full-length cDNA product. Features. Highly thermostable reverse transcriptase 55°C to 65°C and above, Advanced RNase inhibitor, High cDNA yields from as little as 20pg total RNA, Accurate reverse transcription of GC-rich and highly structured transcripts, High sensitivity for challenging templates, Reduced RNase H activity, Available as a stand-alone enzyme with buffer, a cDNA synthesis kit with premixed oligos and a cDNA synthesis kit with separate oligos.
UltraScript 2.0 cDNA Synthesis Kit Separate Oligos
£ 383.00 ex. VATcDNA Synthesis Pack size 100 reactions This modified MMLV reverse transcriptase can be used with reaction temperatures of over 55°C, giving improved specificity, higher cDNA yields and more full-length cDNA product. Features. Highly thermostable reverse transcriptase 55°C to 65°C and above, Advanced RNase inhibitor, High cDNA yields from as little as 20pg total RNA, Accurate reverse transcription of GC-rich and highly structured transcripts, High sensitivity for challenging templates, Reduced RNase H activity, Available as a stand-alone enzyme with buffer, a cDNA synthesis kit with premixed oligos and a cDNA synthesis kit with separate oligos.
UltraScript 2.0 Reverse Transcriptase
£ 184.00 ex. VATcDNA Synthesis Pack size 10,000 units This modified MMLV reverse transcriptase can be used with reaction temperatures of over 55°C, giving improved specificity, higher cDNA yields and more full-length cDNA product. Features. Highly thermostable reverse transcriptase 55°C to 65°C and above, Advanced RNase inhibitor, High cDNA yields from as little as 20pg total RNA, Accurate reverse transcription of GC-rich and highly structured transcripts, High sensitivity for challenging templates, Reduced RNase H activity, Available as a stand-alone enzyme with buffer, a cDNA synthesis kit with premixed oligos and a cDNA synthesis kit with separate oligos.
UltraScript 2.0 Reverse Transcriptase
£ 671.00 ex. VATcDNA Synthesis Pack size 40,000 units This modified MMLV reverse transcriptase can be used with reaction temperatures of over 55°C, giving improved specificity, higher cDNA yields and more full-length cDNA product. Features. Highly thermostable reverse transcriptase 55°C to 65°C and above, Advanced RNase inhibitor, High cDNA yields from as little as 20pg total RNA, Accurate reverse transcription of GC-rich and highly structured transcripts, High sensitivity for challenging templates, Reduced RNase H activity, Available as a stand-alone enzyme with buffer, a cDNA synthesis kit with premixed oligos and a cDNA synthesis kit with separate oligos.
UltraScript cDNA Synthesis Kit
£ 99.00 ex. VATcDNA Synthesis Pack size 25 reactions The enzyme allows highly sensitive reverse transcription of viral RNA sequences, making it an ideal component of RT-qPCR-based detection tests. Available as a stand-alone enzyme, or as a ready mix comprising reverse transcriptase, buffer system and, optionally, with an optimized blend of random hexamers with anchored oligo(dT) primers. This reverse transcriptase provides unbiased, efficient and sensitive cDNA synthesis over a broad range of RNA template concentrations. Features. Unbiased representation of 5′ and 3′ mRNA transcript ends, Reduced RNase H activity, Thermostable reverse transcriptase 45 °C to 55 °C, Advanced RNase inhibitor, High cDNA yields from as little as 4 pg total RNA, Accurate reverse transcription of GC-rich templates, Sensitive detection of low copy number transcripts, Advanced buffer chemistry including Mg and dNTPs, Both premixed and separate oligo cDNA synthesis kits available.
UltraScript cDNA Synthesis Kit
£ 339.00 ex. VATcDNA Synthesis Pack size 100 reactions The enzyme allows highly sensitive reverse transcription of viral RNA sequences, making it an ideal component of RT-qPCR-based detection tests. Available as a stand-alone enzyme, or as a ready mix comprising reverse transcriptase, buffer system and, optionally, with an optimized blend of random hexamers with anchored oligo(dT) primers. This reverse transcriptase provides unbiased, efficient and sensitive cDNA synthesis over a broad range of RNA template concentrations. Features. Unbiased representation of 5′ and 3′ mRNA transcript ends, Reduced RNase H activity, Thermostable reverse transcriptase 45 °C to 55 °C, Advanced RNase inhibitor, High cDNA yields from as little as 4 pg total RNA, Accurate reverse transcription of GC-rich templates, Sensitive detection of low copy number transcripts, Advanced buffer chemistry including Mg and dNTPs, Both premixed and separate oligo cDNA synthesis kits available.
UltraScript cDNA Synthesis Kit Separate Oligos
£ 99.00 ex. VATcDNA Synthesis Pack size 25 reactions The enzyme allows highly sensitive reverse transcription of viral RNA sequences, making it an ideal component of RT-qPCR-based detection tests. Available as a stand-alone enzyme, or as a ready mix comprising reverse transcriptase, buffer system and, optionally, with an optimized blend of random hexamers with anchored oligo(dT) primers. This reverse transcriptase provides unbiased, efficient and sensitive cDNA synthesis over a broad range of RNA template concentrations. Features. Unbiased representation of 5′ and 3′ mRNA transcript ends, Reduced RNase H activity, Thermostable reverse transcriptase 45 °C to 55 °C, Advanced RNase inhibitor, High cDNA yields from as little as 4 pg total RNA, Accurate reverse transcription of GC-rich templates, Sensitive detection of low copy number transcripts, Advanced buffer chemistry including Mg and dNTPs, Both premixed and separate oligo cDNA synthesis kits available.