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Library amplification Pack size 250 reactions A superior proofreading Pfu polymerase in a specially formulated 2x PCR ready mix designed for NGS library amplification with reduced GC bias1. This cutting-edge PCR mix offers market leading performance enabling the acquisition of superior quality datasets with a higher number of unique reads2. The mix can be combined with our library quantification kit during NGS library preparation. Features. Low GC bias, ideal for high GC/AT targets, more unique reads per NGS dataset for superior data quality, AptaLock? hot start technology for maximum sensitivity and specificity, 100x higher fidelity than Taq DNA polymerase, Room temperature setup, 2x ready mix for minimal pipetting.

Library amplification Pack size 50 reactions A superior proofreading Pfu polymerase in a specially formulated 2x PCR ready mix designed for NGS library amplification with reduced GC bias1. This cutting-edge PCR mix offers market leading performance enabling the acquisition of superior quality datasets with a higher number of unique reads2. The mix can be combined with our library quantification kit during NGS library preparation. Features. Low GC bias, ideal for high GC/AT targets, more unique reads per NGS dataset for superior data quality, AptaLock? hot start technology for maximum sensitivity and specificity, 100x higher fidelity than Taq DNA polymerase, Room temperature setup, 2x ready mix for minimal pipetting.

Library quantification Pack size 85 ?L each The kit uses qPCR to specifically quantify adapter-ligated DNA molecules, ensuring optimal cluster densities for improved sequencing efficiency and quality of data. Features. Uses qPCR to accurately and rapidly quantify a library prior to sequencing , Gives consistent library quantification across a wide range of sample types, concentrations, fragment sizes and GC content, Uses a single extension time for all libraries , Allows specific quantification of only DNA molecules that can be sequenced by NGS , Uses antibody-mediated hot start technology to ensure all reactions start simultaneously, Compatible with all Illumina instruments and qPCR platforms, Suitable for manual and automated workflows , Available as an easy-to-see blue qPCR mix , Easily calculate library concentration with the online NGSBIO Library Quantification Tool.

Library quantification – with qPCRBIO SyGreen Blue Separate-ROX Pack size 500 reactions The kit uses an easy-to-see blue qPCR mix to specifically quantify adapter-ligated DNA molecules, ensuring optimal cluster densities for improved sequencing efficiency and quality of data. Features. Easy-to-see blue mix for greater pipetting precision and accuracy, uses qPCR to accurately and rapidly quantify a library prior to sequencing, gives consistent library quantification across a wide range of sample types, concentrations, fragment sizes and GC content, uses a single extension time for all libraries, allows specific quantification of only DNA molecules that can be sequenced by NGS, Uses antibody-mediated hot start technology to ensure all reactions start simultaneously, Compatible with all Illumina instruments and qPCR platforms, Suitable for manual and automated workflows, Easily calculate library concentration with the online NGSBIO Library Quantification Tool.

Library quantification – with qPCRBIO SyGreen Blue Separate-ROX Pack size 100 reactions The kit uses an easy-to-see blue qPCR mix to specifically quantify adapter-ligated DNA molecules, ensuring optimal cluster densities for improved sequencing efficiency and quality of data. Features. Easy-to-see blue mix for greater pipetting precision and accuracy, uses qPCR to accurately and rapidly quantify a library prior to sequencing, gives consistent library quantification across a wide range of sample types, concentrations, fragment sizes and GC content, uses a single extension time for all libraries, allows specific quantification of only DNA molecules that can be sequenced by NGS, Uses antibody-mediated hot start technology to ensure all reactions start simultaneously, Compatible with all Illumina instruments and qPCR platforms, Suitable for manual and automated workflows, Easily calculate library concentration with the online NGSBIO Library Quantification Tool.

Library quantification – with qPCRBIO SyGreen Blue Hi-ROX Pack size 500 reactions The kit uses an easy-to-see blue qPCR mix to specifically quantify adapter-ligated DNA molecules, ensuring optimal cluster densities for improved sequencing efficiency and quality of data. Features. Easy-to-see blue mix for greater pipetting precision and accuracy, uses qPCR to accurately and rapidly quantify a library prior to sequencing, gives consistent library quantification across a wide range of sample types, concentrations, fragment sizes and GC content, uses a single extension time for all libraries, allows specific quantification of only DNA molecules that can be sequenced by NGS, Uses antibody-mediated hot start technology to ensure all reactions start simultaneously, Compatible with all Illumina instruments and qPCR platforms, Suitable for manual and automated workflows, Easily calculate library concentration with the online NGSBIO Library Quantification Tool.

Library quantification – with qPCRBIO SyGreen Blue Hi-ROX Pack size 100 reactions The kit uses an easy-to-see blue qPCR mix to specifically quantify adapter-ligated DNA molecules, ensuring optimal cluster densities for improved sequencing efficiency and quality of data. Features. Easy-to-see blue mix for greater pipetting precision and accuracy, uses qPCR to accurately and rapidly quantify a library prior to sequencing, gives consistent library quantification across a wide range of sample types, concentrations, fragment sizes and GC content, uses a single extension time for all libraries, allows specific quantification of only DNA molecules that can be sequenced by NGS, Uses antibody-mediated hot start technology to ensure all reactions start simultaneously, Compatible with all Illumina instruments and qPCR platforms, Suitable for manual and automated workflows, Easily calculate library concentration with the online NGSBIO Library Quantification Tool.

Library quantification – with qPCRBIO SyGreen Blue Lo-ROX Pack size 500 reactions The kit uses an easy-to-see blue qPCR mix to specifically quantify adapter-ligated DNA molecules, ensuring optimal cluster densities for improved sequencing efficiency and quality of data. Features. Easy-to-see blue mix for greater pipetting precision and accuracy, uses qPCR to accurately and rapidly quantify a library prior to sequencing, gives consistent library quantification across a wide range of sample types, concentrations, fragment sizes and GC content, uses a single extension time for all libraries, allows specific quantification of only DNA molecules that can be sequenced by NGS, Uses antibody-mediated hot start technology to ensure all reactions start simultaneously, Compatible with all Illumina instruments and qPCR platforms, Suitable for manual and automated workflows, Easily calculate library concentration with the online NGSBIO Library Quantification Tool.

Library quantification – with qPCRBIO SyGreen Blue Lo-ROX Pack size 100 reactions The kit uses an easy-to-see blue qPCR mix to specifically quantify adapter-ligated DNA molecules, ensuring optimal cluster densities for improved sequencing efficiency and quality of data. Features. Easy-to-see blue mix for greater pipetting precision and accuracy, uses qPCR to accurately and rapidly quantify a library prior to sequencing, gives consistent library quantification across a wide range of sample types, concentrations, fragment sizes and GC content, uses a single extension time for all libraries, allows specific quantification of only DNA molecules that can be sequenced by NGS, Uses antibody-mediated hot start technology to ensure all reactions start simultaneously, Compatible with all Illumina instruments and qPCR platforms, Suitable for manual and automated workflows, Easily calculate library concentration with the online NGSBIO Library Quantification Tool.