VeriFi Hot Start Mix

VeriFi Hot Start Polymerase is a single enzyme derived from Pfu DNA polymerase for its 3?-5? exonuclease (proofreading) activity. Proprietary mutations improve DNA binding and increase processivity when compared to its native form, resulting in shorter extension times, higher yields, and the ability to amplify longer and more difficult targets. VeriFi Hot Start Polymerase is able to amplify eukaryotic genomic templates in excess of 17.5 kb, and longer for simpler DNA templates. Extension times of 30 s per kb are recommended for most applications. PCRBIO?s innovative AptaLock? technology uses a proprietary aptamer-like molecule that reversibly inhibits both the 3?-5? exonuclease activity and 5?-3? polymerase activity of the enzyme below 40?C. This unique hot start molecule prevents primer dimer formation and non-specific amplification to maximize the sensitivity and specificity of your PCR. This feature makes VeriFi Hot Start Polymerase highly suitable for multiplexing and enables reactions to be set up at room temperature. The reaction mix is stable both before and after the PCR run for up to 24 hours, meaning greater ease of use for high throughput assays. The enhanced accuracy of VeriFi Hot Start Polymerase gives extremely low error rates and fidelity that is approximately 100 times higher than Taq DNA polymerase. The enzyme is ideal for applications where superior accuracy is required, such as cloning, site-directed mutagenesis, and sequencing. The enzyme is provided with an advanced buffer system including dNTPs, Mg, and enhancers, enabling high-fidelity PCR of a wide range of targets and fragment sizes regardless of GC or AT content. For the most difficult templates, such as highly GC-rich sequences or those with complex secondary structures, users have the option of adding the VeriMax Enhancer to the reaction mix for improved PCR performance. VeriFi Hot Start Polymerase is also available as a convenient 2x ready mix containing all reaction components apart from primers and template. Our 2x red mix contains a red dye suitable for direct loading and tracking during agarose gel electrophoresis.