UltraScript Reverse Transcriptase

UltraScript Reverse Transcriptase is a thermostable and extremely active modified MMLV reverse transcriptase (RTase). The reaction temperature can be increased up to 55°C providing higher specificity and efficient transcription of GC-rich RNA regions with a high secondary structure. The RTase is blended with an advanced RNase inhibitor preventing degradation of RNA by contaminating RNase. UltraScript Reverse Transcriptase is not inhibited by ribosomal and transfer RNAs, making total RNA an ideal substrate. The enzyme is supplied with a 5x buffer containing Mg, dNTPs, stabilizers and enhancers, optimized to generate high yield cDNA from as little as 4 pg RNA. As oligos are not included, UltraScript Reverse Transcriptase provides the convenience and flexibility for users to define their own priming strategy depending on the type of analysis needed. UltraScript Reverse Transcriptase gives exceptional performance with gene-specific primers, oligo(dT) and random hexamers to produce high quality cDNA ideal for a variety of downstream applications. Our powerful RTase is also available in high concentration formats, and as ready-made UltraScript cDNA Synthesis Kit (formerly qPCRBIO cDNA Synthesis Kit), in which the relative concentrations of random hexamers and anchored oligo(dT) have been optimized for the generation of cDNA for use in real-time PCR experiments. This eliminates the need for user optimization of the critical reverse transcription reaction factors, such as priming strategy, reverse transcriptase, buffer and enhancers, and gives unbiased, efficient and sensitive cDNA synthesis every time.