RiboShield RNase Inhibitor

Ribonucleases (RNases) are found in all cell types and organisms, and their ubiquitous nature can be highly problematic in the lab. Contamination with even the smallest amount of RNase in an RNA sample is enough to completely destroy the RNA. RiboShield RNase Inhibitor is a recombinant human placental protein that blocks the activity of a wide range of ribonucleases, including RNase A, B and C, to reliably protect your RNA from RNase digestion. RiboShield RNase Inhibitor is designed for RNA-sensitive applications including RNA isolation, RT-qPCR, cDNA synthesis, and RNA sequencing, to shield your RNA from degradation and provide higher yields and better performance as a result. The inhibitor binds noncovalently to RNases at a 1:1 ratio and with very rapid kinetics of association, guaranteeing immediate protection of your RNA. RiboShield RNase Inhibitor has been tested in RT-qPCR and offers the greatest RNA protection when compared to leading competitor products. RiboShield RNase Inhibitor is able to perform over a wide range of reaction conditions and can sustain inhibition of RNase A at temperatures up to 65°C for at least 30 minutes (see figure 2). In addition, RiboShield does not contain cysteine residues that have been implicated in the oxidation sensitivity of the human placental version of the protein1. This results in an RNase inhibitor molecule that is not only thermostable, but also more resistant to oxidative stress. The inhibitor can be used to block the activity of a wide range of ribonucleases, including eukaryotic RNases of the neutral type (RNases A, B and C). It does not inhibit RNases T1, T2, U1, U2, CL3, RNase I and H. The inhibitor does not hinder other enzymes such as reverse transcriptases, RNA polymerases or Taq DNA polymerase making it compatible with many enzymatic reactions involving RNA. RiboShield RNase Inhibitor has a molecular weight of 50 kDa and is purified from High Five insect cells expressing a modified human placental gene. The inhibitor binds noncovalently to RNases at a 1:1 ratio and has a Ki value of approximately 10?14 M when binding to RNase A1. Moreover, the very rapid kinetics of association to RNases guarantees immediate protection of your RNA.