Clara Probe Mix Hi-ROX

Clara Probe Mix is engineered to enable qPCRs with the highest sensitivity, reliability, and greatest ease of use in diagnostic applications and basic research alike. It is a universal qPCR mix suitable for all types of probe technologies, including TaqMan?, Scorpions?, and molecular beacons. Powered by our unique hot start Taq DNA polymerase the qPCR mix is suitable for DNA detection for 2-step RT-qPCR protocols when used with a separate cDNA synthesis kit. Although for routine RNA quantification, we recommend 1-step protocols with Clara Probe 1-Step Mix for a more streamlined workflow. Clara Probe Mix can be used for all types of probe-based qPCR applications, including gene expression analysis, SNP/allele detection, genotyping and allelic discrimination studies, and species abundance quantification, and can offer high efficiency in both single and multiplex assays. Making this mix highly versatile and ideal for in-vitro diagnostic kit development as well as basic research. The mix is also suitable for melt-curve analysis (with hybridization probes only) and is available without passive reference dye (No-Rox), or with reverence dye as Lo-ROX, Hi-Rox, and separate-ROX formulations.