Life Science Applications

Temperature control equipment

Cole-Parmer has an entire range of lab products dedicated to the manufacture of temperature control. This range of science equipment is very specific to the particular development of controlling temperature and includes, including water baths, Dri-block heaters and molecular biology products such as hybridization incubators and thermal cyclers.

  • Baths & Thermoregulators
  • Biological Stirrers
  • Block Heaters
  • Gelation Timers
  • qPCR Detection
  • Sample Cooling
  • Shakers and Rockers
  • Thermal Cycling
  • Water Baths
  • Brochure HERE

Life science spectrophotometer

The MESLO M-Drop XF Nano Spectrophotometer provides quick and easy quantification of small samples. Utilising a small sample volume eliminates the need for dilutions, providing more accurate results. A full colour, 7”’touchscreen with icon-driven menus and preprogramed protocols gets users up and running quickly. No external computer is required. Each unit features a quartz pedestal onto which a drop of sample is placed. The arm is lowered and the drop is stretched into a liquid column of specified length for spectral analysis. DNA, RNA and protein concentrations and purity numbers are displayed in a matte
of seconds.

  • Full-spectrum absorbance scanning and calculations of
    260/280 and 260/230 ratios.
  • Long-life Xenon lamp with precision monochromator.
  • Auto-ranging path lengths of 0.05mm, 0.2mm and 1.0mm.
  • Detect up to 15,000ng/ul of dsDNA without dilution.
  • Integrated Fluorometer for sensitive quantitation of low
    concentration nucleic acids.

Measure sample volumes as low as 0.5 μL with a high degree of accuracy and reproducibility in less than 6.5 seconds!
  • Preprogrammed methods for DNA, RNA and Protein measurements
  • Detects DNA concentrations as low as 2 ng/μL
  • Icon-driven software and easy-to-use navigation system provide intuitive usability
  • Method and result saving to USB memory stick
  • Easy and quick to clean

Automated nucleic extraction systems

The purification of DNA or RNA is an early and mandatory step in many life science processes, used to detect bacteria and viruses in environmental, forensic, or clinical samples used to diagnose diseases, cancer or genetic disorder. Furthermore, automated nucleic acid extractor designed for small, medium and high throughput applications. Specialized spin tips enable efficient mixing of magnetic beads

The basic procedure of our nucleic acids isolations:

  • Lysis of cell membrane
  • Denaturation of proteins, DNA and other macromolecules
  • Promotion of nucleic acid binding to magnetic particles
  • Removal of impurities​
  • Collection of purified DNA / RNA

The fully automated magnetic bead ope

Automated Nucleic Acid Purification, continuously help to improve laboratory workflows and to reduce human error.

Tanbead Automated Extraction Instruments

The fully automated magnetic bead operation platforms use magnetic rods in the equipment to isolate nucleic acid, and the magnetic beads adsorbed with nucleic acids are automatically transferred from one well to another for cell lysis, nucleic acid adsorption, washing, and elution. Compared with complex manual spin column operations, TANBead’s automated extraction devices provide you with a walk-away solution and labor-saving method for processing multiple samples at the same time.

  • Tanbead Maelstrom Switch 8 Changeable gearbox match TANBead’s 96-deepwell, 24-deepwell and auto-tube
    Consumable parts support sample volume for diferent needs Fool-proof design refrain from operation error.
  • Maelstrom 4810 Process up to 48 samples per run Efficient purifcation driven by TANBead patented technology Covid extraction only takes about 15 minutes
  • Maelstrom 9610 High-throughput: 96 samples/run, Efficient whirl stir mixing approach, Speed and precision: 4 independent temperature control modules, Flexibility in samples/run: 6, 12 , 18 , 24 , 30 , 36 up to 48 (with single tube)
  • Maelstrom 2410 Automated solutions that reduce complex manual steps. Efficient purification driven by TAN Bead patented technology. Large processing volume up to 9 ml Prevent the risk of cross-contamination caused by aerosol generation.

These reagent kits are suitable for nucleic acid extraction from virus samples (such as: swabs, blood, saliva, etc.). The purified nucleic acid can be used for polymerase chain reaction technology (PCR/ qPCR) to detect nucleic acid molecules in the samples.

Viral Nucleic Acid Extraction, 665 series

  • Applicable to a variety of samples with low viral load.
  • Rapid and automated nucleic acid extraction with a choice of different throughputs.
  • Can be directly applied to downstream detection assays.

Universal Pathogen Extraction, 66P series RUO

  • Applicable to pathogenic microorganism extraction.
  • Rapid and automated nucleic acid extraction with a choice of different throughputs.
  • Can be applied to downstream application directly

Virapid Viral Nucleic Acid Extraction, 685 series CE-IVD & RUO

  • Applicable to a variety of samples.
  • Rapid and automated nucleic acid extraction without additional pre-processing.
  • Can be directly applied to downstream detection assays.

HBV DNA Extraction, 615 series CE-IVD & RUO

  • Can extract nucleic acid from samples with low virus concentration.
  • Rapid and automated nucleic acid extraction with a choice of different throughputs.
  • Can be directly applied to downstream detection assays.

HPV DNA Extraction, 61H series RUO

  • Simple pre-procesiing of highly viscous samples.
  • Rapid and automated nucleic acid extraction with a choice of different throughputs.
  • Can be directly applied to downstream detection assays.

Viral Nucleic Acid Extraction, 635 series CE-IVD & RUO

  • Viral nucleic acid can be extracted from serum and plasma.
  • Rapid and automated nucleic acid extraction with a choice of different throughputs.
  • Can be directly applied to downstream detection assays.

QPCR real time PCR, kits, and qPCR workflow

DNA/RNA purification instruments and reagent products for life science, research and clinical diagnosis.

 

Our Comprehensive Solutions for Your Laboratory:

  • Endpoint PCR Workflow
  • NGS Workflow
  • REAL TIME PCR Workflow
  • Brochure HERE

AZURE CIELO™ Real-Time PCR

Offering innovative optics and technology for high-performance real-time PCR:

  • Flexibility through innovation
  • Sensitivity by design
  • Engineered for faster run times
  • Built for reproducibility
  • Simplicity through software

The Azure Cielo Real-Time PCR system is available in either a 3-channel or 6-channel configuration and is compatible with a wide spectrum of qPCR dye-based and probe-based chemistries. Azure Cielo was designed around individual well scanning, with a scan head comprised of two sets of 16 fiber optics. Channel-specific excitation and emission light emitting diode (LED) light sources ensure that specific wells on the qPCR plate are illuminated, increasing sensitivity and reducing background noise. Up to 6 fluorescent channels allow for flexible dye usage and broad application. Additionally, Rox isn’t required, so you can use it as a probe.

The Cole-Parmer PCR-300 Alpha Cyclers

Developed to deliver not only the best quality data you can expect from a thermal cycler but also to innovate and exceed the high standards expected by the community. With options of either one, two or four independently controllable blocks, the PCR-300 is a platform which scales to the throughput of the laboratory. With programs easily transferable between any PCR-300 instrument and user specific defaults stored on the user’s USB login device (any USB drive can be programmed as a login device) there are no issues with transferring work between systems or collaborating/sharing across multiple sites Our specialized equipment optimizes:

  • Android driven HD touchscreen
  • Automated protocol optimization with program wizard
  • Remote run and system monitoring via the application
  • Authorize any USB drive to be the user’s login key
  • Lock, protect and create customized programs for ease of access and security
  • 96 to 384-well blocks available with block types having gradient functionality
  • Manually adjustable heated lids

DNA Internal Control- The kit is supplied with an exogenous source of DNA which can be spiked into the lysis buffer and co-extracted with the sample of interest.

RNA Internal Control – The kit is supplied with an exogenous source of RNA which can be spiked into the lysis buffer and co-extracted with the sample of interest.

YouSeq Lyophilised Tetra™ 2X qPCR MasterMix uses unique enzymes and proprietary additives to guarantee a powerful, inhibitor tolerant qPCR reaction.

YouSeq Lyophilised Tetra™ OneStep 2X qRT-PCR MasterMix uses unique enzymes and proprietary additives to guarantee a powerful, inhibitor tolerant qPCR reaction.

YouSeq Tetra™ 2X qPCR MasterMix uses unique enzymes and proprietary additives to guarantee a powerful, inhibitor tolerant qPCR reaction.

YouSeq Tetra™ OneStep 2X qRT-PCR MasterMix uses unique enzymes and proprietary additives to guarantee a powerful, inhibitor tolerant qPCR reaction.

Flourescence Microplate Reader

LTEK Flourescence Microplate Reader Inno-S

  • Calcium Assay (GPCR)
  • Caspase-3 apoptosis Assay
  • Cell Growth Assay
  • Cytotoxicity Assay
  • Fluorescent protein quantification
  • Nucleic Acid quantification

Electrophoresis

A complete range of Gel Electrophoresis systems:

Equipment for concentrating viruses from liquids

The Innovaprep Concentrating Pipette (CP) Select™, uses dead-end filtration on high-flow single-use pipette tips to capture target particles. The Innovaprep CP Select uses an automated recovery process, termed, Wet Foam Elution™ to enable efficient extraction of the particles from the filter membrane in seconds and can administer very precise and controlled amounts of foam that produce filter elution volumes as low as 200 microliters. The process maximizes the concentration of the target thereby exponentially improving the limit of detection for trace pathogens.

InnovaPrep’s FluidPrep™ technologies including the Large Volume Concentration (LVC) Kit and the CP Select have been used to concentrate microorganisms including viruses in liquid matrices from a variety of environmental sources such as marine recreational and aquaculture waters, surface waters, storm run-off, cave and spring waters, irrigation waters, as well as wastewater influent and effluent. The Concentrating Pipette pairs with perfectly with most molecular methods including PCR and sequencing.

Wastewater Based Epidemiology using the CP Select

Public health labs, testing services labs, universities, and the military are using wastewater as a means to detect the emergence or spread of COVID-19 outbreaks which has proven to be a significant epidemiological method to help determine where to mobilize resources and determine the degree and duration of intervention needed in a community by community real-time approach.

The Concentrating Pipette effectively concentrates SARS-CoV-2 from wastewater influent faster, easier, and better than other methods by improving the limit of detection, reducing inhibition, simplifying workflow, and by reducing labor. The instrument fits into BSL-2 safety cabinets for increased ease and safety.