Clara Probe 1-Step Mix Separate-ROX

Clara Probe 1-Step Mix enables qPCR and 1-step RT-qPCR with the highest sensitivity, reliability, and with the greatest ease of use in diagnostic applications and basic research alike. It is a universal qPCR mix suitable for all types of probe technologies, including TaqMan?, Scorpions? and molecular beacons. This is our first generation of all-in-one 1-step RT-qPCR mixes, where both the DNA polymerase and reverse transcriptase are included in the same mastermix. Clara Probe 1-Step Mix is powered by our unique hot start Taq DNA polymerase and a specially modified version of our high performance, thermostable UltraScript Reverse Transcriptase. Rigorous testing during development makes this mix highly suitable for both single and multiplex detection with high efficiency and sensitive detection whether you?re detecting one target or many simultaneously. Our proprietary buffer composition (identified via smart screen technology) and uniquely modified UltraScript RTase allow the mix to be stored in a single tube, as all-in-one, RT-qPCR mastermix. This reduces the amount of pipetting needed for assay setup, minimizing the risk of contamination, and saving time. It also means that one reagent is suitable for amplification of DNA and RNA and both target types can be included on the same plate or in the same run. Having one mix means you do not require a different setup when primarily investigating RNA targets, but still requiring DNA quantification. While users who test large quantities of both target types of targets may prefer dedicated reagents for each target type, Clara Probe Mix is developed exclusively for DNA and cDNA probe-based detection. Extensive optimization makes this mix suitable for all nucleic acid target types. We have tested it against common RNA viruses, including SARS-CoV-2, RSV, Influenza A, and B, standard housekeeping genes, such as g-actin and GAPDH, as well as DNA targets. The presence of RTase in the mix does not affect direct amplification of DNA targets, meaning you get the same high efficiency whether you?re detecting DNA or RNA sequences. The mix is highly stable and shows strong reproducibility between runs, giving your data reproducibility and confidence in the experimental conclusions you draw. Extensive optimization makes this mix suitable for all nucleic acid target types. We have tested it against common RNA viruses, including SARS-CoV-2, RSV, Influenza A, and B, standard housekeeping genes, such as g-actin and GAPDH, as well as DNA targets. The presence of RTase in the mix does not affect direct amplification of DNA targets, meaning you get the same high efficiency whether you?re detecting DNA or RNA sequences. The mix is highly stable and shows strong reproducibility between runs, giving your data reproducibility and confidence in the experimental conclusions you draw.