qPCRBIO Probe 1-Step Go is a universal probe kit designed for fast, highly specific, and sensitive probe-based real-time RT-PCR. We use the latest developments in reverse transcriptase technology and buffer chemistry to give efficient cDNA synthesis and real-time PCR in a single tube. qPCRBIO Probe 1-Step Go is engineered for use on a wide range of probe technologies including TaqMan?, Scorpions? and molecular beacons probes. The kit includes a 2x mix and can be used to quantify any RNA template including mRNA, total RNA, and viral RNA sequences. qPCRBIO Probe 1-Step Go is designed to give rapid and accurate results over a broad range of template concentrations and is ideally suited to the detection of RNA viruses including SARS-CoV-2. The kit includes a thermostable and extremely active modified MMLV reverse transcriptase (RTase Go) and advanced RNase inhibitor that prevents the degradation of RNA by contaminating RNase. Antibody-mediated hot start technology prevents the formation of primer dimers and non-specific products giving highly specific and ultra-sensitive real-time RT-PCR with unrivalled efficiency in multiplex. Combining the latest developments in polymerase technology and advanced buffer chemistry we offer market-leading performance with minimal or no optimisation.
qPCRBIO Probe 1-Step Go Hi-ROX
qPCR From RNA – 5 mL bottle Pack size 500 x 20 ?L rxns The kit is engineered for use on a wide range of probe technologies including TaqMan?, Scorpions? and molecular beacon probes. Features. Rapid and sensitive detection of RNA viruses including SARS-CoV-2, Rapid extension rate for early Ct values, Market-leading sensitivity ? increased limit of detection, High efficiency in multiplex reactions, Advanced RNase inhibitor, Antibody-mediated hot start PCR, Compatible with all real-time PCR platforms ? standard and fast cycling conditions.
SKU:
PB25.42-05
Category: qPCR Kits
Features
Specifications
Parameter | Value |
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Brand | |
Product type | Probe 1-Step Go |
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About brand
PCR Reagents for Research & Diagnostics:
PCR Biosystems develops a range of PCR reagents that maximise yield and sensitivity from the simplest to most challenging of reactions. Their products outperform every competitor in terms of yield, specificity, sensitivity, and speed. Applications include PCR, qPCR, NGS, Molecular Diagnostics, cDNA Synthesis, and DNA Extraction.