VeriFi Mix

VeriFi Polymerase is derived from Pfu DNA polymerase for its 3?-5? exonuclease (proofreading) activity. The enzyme is engineered with proprietary mutations that significantly increase processivity, resulting in shorter extension times (10-30 s/kb), higher yields, and the ability to amplify longer and more difficult targets, including eukaryotic genomic templates in excess of 17.5 kb. The high accuracy and enhanced 3?-5? exonuclease activity of VeriFi Polymerase results in fidelity that is approximately 100 times higher than Taq DNA polymerase. The enzyme is ideally suited to applications where greater accuracy is required, such as cloning, site-directed mutagenesis, and sequencing. PCR products generated with this range of products are blunt-ended. VeriFi Polymerase enables high-fidelity PCR of a wide range of targets and fragment sizes with minimal or no optimization required. The enzyme is provided with an advanced buffer system including both dNTPs and Mg. For the most difficult templates, such as highly GC-rich sequences or those with complex secondary structures, users have the option of adding the VeriMax Enhancer to the reaction mix for improved PCR performance. VeriFi Polymerase is also available as a convenient 2x ready mix containing all reaction components apart from primers and templates. Our 2x red mix contains a red dye suitable for direct loading and tracking during agarose gel electrophoresis.