Cleaver Scientific is a world-leading supplier of Electrophoresis systems and related laboratory products.
Drawing on years of manufacturing and laboratory experience, Cleaver Scientific is a UK based electrophoresis equipment supplier providing premium and technically enhanced, high specification, low-cost electrophoresis equipment for the world market. The product range includes DNA electrophoresis units, protein electrophoresis units, Electroblotters, Radiation protection and Gel Documentation Equipment. Cleaver Scientific is based in Rugby, Warwickshire, in the centre of the UK. Cleaver Scientific premises are well served by both rail and road networks.
**Cleaver Scientific is an ISO9001:2008 accredited company and all their products comply with CE regulations.
How does Electrophoresis work?
An electric field is applied to molecules and as they are electrically charged themselves it results in a force acting upon them. The greater the charge of the molecule the greater the force applied by the electrical field and therefore the further through the support medium the molecule will move relative to its mass.
Some example applications of electrophoresis include DNA and RNA analysis as well as protein electrophoresis which is a medical procedure used to analyse and separate the molecules found in a fluid sample (most commonly blood and urine samples).
Types of electrophoresis
Different types of gels are usually used as the support medium for electrophoresis and this may be in slab or tube form depending on which is more beneficial. Gel slabs enable many samples to be run simultaneously and so are frequently used in laboratories. However, tube gels give a better resolution of the results so are often chosen for protein electrophoresis.
Agarose gel is commonly used for electrophoresis of DNA. It has a large pore structure allowing larger molecules to move easily but it is not suitable for sequencing smaller molecules.
Polyacrylamide gel electrophoresis (PAGE) has a clearer resolution than agarose gel making it more suitable for quantitative analysis. This makes it possible to identify how proteins bind to DNA. It can also be used to develop the understanding of how bacteria is becoming resistant to antibiotics through plasmid analysis.
2D Electrophoresis separates molecules along an x-axis and a y-axis – one separating them by charge and the other by size.